Units HOEFER
1.SE250-10A.-75 Mighty small II complete, mini vertical electrophoresis unit HOEFER:
Designed for quick screening and rapid separaton
chnic al Specif ic at ions
Glass Plate Size (w x h) . . . . . . . 10 x 8 cm
Spacers . . . . . . . . . . . . . . . . . . . 0.75 m
m, 1.0 mm, 1.5 mm
Maximum Power Settings . . . . . 500 V, 500 mA, 12 W
Maximum Temperature . . . . . . . 45°C
Indoor Use . . . . . . . . . . . . . . . . 4-40°C
Humidity . . . . . . . . . . . . . . . . . Up to 80%
Unit Dimension (w x h x d) . . . . 16.5 x 16 x 16 cm
Safety Certifications . . . . . . . . . EN61010-1, UL
61010A-1, CSA C22.2 1010.1, CE
2.TE22 Mighty small transphor HOEFER:
Quickly and efficiently transfers proteins and nucleic acids from small gels to nylon, nitrocellulose or PVDF membranes
Maximum Gel Size 9 x 10 cm
• Transfers as many as four small gels, up to 9 x 10 cm in less than an hour
• Built-in alumina-covered cooling channel provides excellent temperature control with no more than a 5°C temperature increase during a typical run
• Superior design provides a uniform electric field-the key to even transfers
• Cassettes are easy to load and handle
• No gel distortion-grooves inside the unit hold cassettes in place, applying equal pressure across the gel
3. SE300-10A-1.0 miniVE Integrated Vertical Electrophoresis Unit HOEFER:
Perform both electrophoresis and electro transfer in one sturdy, compact unit
• Lower Buffer Chamber (tank)
• Gel Modules with Gaskets-2 assemblies
• Safety Lid with High Voltage Leads
• Glass Plates, Rectangular, 10 x 10.5 cm-3 pcs
• Glass Plates, Notched, 10 x 10.5 cm-3 pcs
• Combs, 10-well, 1.0 mm thick-2 pcs
• T-Spacers, 1.0 mm thick-4 pcs
• Well-Locating Decals-2 pcs
• Wonder Wedge Plate Separation Tool
• Gel Seal
4.SE 302 Mini VE Blotter HOEFER:
Transfer module for mini VE. Includes 3 Dacron™ sponges 6.35mm thick, 25 sheets of blotter paper
5.TE77XP Semi-Dry Transfer Units with Built-In Power Supply HOEFER:
Unique design that includes an intelligent built-in power supply which automatically monitors the transfer status, stopping the transfer before the stack overheats
The Semi-Dry Transfer Units support as many as two layers of gels being transferred simultaneously. The TE70XP has a 14 x 16 cm maximum transfer area which can handle up to four mini gels using the stacked format. The TE77XP has a 21 x 26 cm transfer area for a capacity of up to 12 mini gels at one time.
6. TE77X Semi-Dry Transfer Units with Built-In Power Supply HOEFER:
The Semi-Dry Transfer Units support as many as two layers of gels being transferred simultaneously. The TE70XP has a 14 x 16 cm maximum transfer area which can handle up to four mini gels using the stacked format. The TE77XP has a 21 x 26 cm transfer area for a capacity of up to 12 mini gels at one time.
L10001 Luna automated cell counter HOEFER: Luna™ automated cell counter gives you the accurate live/dead cell counting results within 7 seconds. Based on the precision optical design and the new software algorithm, the cell counting accuracy is unsurpassed. Within a 5X104 ~ 1X107 cells/ml concentration range and 3 ~ 60 ㎛ cell diameter, Luna™ software accurately detects live/dead cells, and discriminates cell debris. Individual cells in cell clusters are also successfully counted by Luna’s new de-clustering algorithm.
Cell counter (Luna Automated Cell Counter): Automatized cell counter is used for quick cell counting. This is especially useful when 15-20 samples are studied at the same time. The cell counter is also able to determine the ratio of live and dead cells.
Gel electrophoresis apparatus (Mighty small II complete): The electrophoresis apparatus is used to separate proteins isolated from cell lysates by charge or size. Westernblot techniques help to study our separated protein sample lysated after cell treatment. These new treatments make possible the understand the cellular features.
Gel electrophoresis apparatus (Mini VE complete): The electrophoresis apparatus is used to separate proteins isolated from cell lysates by charge. Westernblot techniques help to study our separated protein sample lysated after cell treatment. These new treatments make possible the understand the cellular features.
Equipment for transfer (Mighty small transphor with power supply, Mighty small transphor without power supply, chamber for wet blotting): For further study of the gels the samples are transferred to membranes. Both wet and semi-dry transfers are used for our experiments. The semi-dray transfer is required for small proteins (10-75 kDa), while the wet transfer is essential for transferring large proteins (75-250 kDa).
Semmelweis Egyetem, Elméleti Orvostudományi Központ,
Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézet,
2. floor, 2.117. labor
Contact: Dr. Bánhegyi Gábor, banhegyi.gabor@med.semmelweis-univ.hu, 266 26 15/60100
Gábor Bánhegyi - Csaba Sőti - Tibor Vellai