Shape and compactness of proteins studied by a combined NMR - SAXS approach
Changes in protein shape have an effect on their biological function. In order to clarify these variations induced by folding, an in-depth structural characterization is indispensable. The combined application of NMR spectroscopy and small-angle X-ray scattering (SAXS) provides a unique possibility for determining the shape and compactness of proteins in biologically relevant environment (solution state). The synergy of the two methods also enables us to study membrane proteins by in vitro model systems (bicelles), equivalent to their natural lipid environment.
Andrea Bodor - Attila Bóta