The eighth Conference of the MedInProt Protein Science Research Synergy Program was held on April 21. 2018, at Eötvös Loránd University.
Gergő Gógl: A crystallographer’s perspective on cryo-electron microscopy
The role of ion channels and pumps in tumor metastasis
Interaction of lysophosphatidic acid with signalling protein domains: affinity, stoichiometry and the site of binding
Role of the scaffold protein Tks4 in cancer cell movement
Setup of inflammation induced permeability measurements by gold nanoparticles and optical biosensor
Pathogenicity and oligomerization of podocin
Studying decision making process between cell death mechanisms: the GSH switch - ferroptosis and autophagy in focus
Investigation of the role of dynamin mediated and mitochondrial pathways in the background of centronuclear myopathy
Mihály Pósfai: New scientific directions in electron microscopy laboratory
Ferenc Vonderviszt: The revolution of cryo-electron microscopy in determination of biomolecular structures
Recent advances in cryo-electronmicroscopy have dramatically reshaped the landscape of structural biology. This technique can be used to determine the three-dimensional structure of biomacromolecules in near native conditions at close to atomic resolution, and has the potential to reveal multiple conformational states related to the dynamic behavior of molecular complexes. Cryo-EM now produces very excit¬ing results addressing a broad range of important biological questions with a level of detail we have never seen before.Ágnes Hubert: From protein sample to high-resolution cryo-electron microscopy map
Cryo-electron microscopy (cryo-EM), is an increasingly popular technique allowing the examination of a frozen-hydrated specimen in vitreous (non-crystalline) ice. The technique complements X-ray crystallography by revealing structural details without the need for a crystalline specimen. The workflow of single-particle cryo-EM is demonstrated with the case-study of three archaeal protein complexes and the structure of the eukaryotic 26S proteasome.Gergő Gógl: A crystallographer’s perspective on cryo-electron microscopy
I will discuss the advantages and the potential problems of cryo EM modelling in constrast to the conventional crystallographic structure determination.
László Nyitray: Resolution revolution
The Nobel prize in chemistry in 2017 was awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for developing cryo-electron microscopy as a structural biology method. In the lecture the development of the Method of the Year in 2015 will be briefly described together with the contribution of the Nobel prize winners and further methodological progress in the last couple of years that explains the "resolution revolution".
Max Maletta: How Cryo-EM is changing the Structural Biology landscape for the best
In 2017 R. Henderson, J. Frank and J. Dubochet have been awarded the Nobel prize in Chemistry for having pioneered cryo electron microscopy (Cryo-EM) and Single Particle Analysis (SPA).
During the last few years Cryo-EM and SPA have grown from techniques able to produce low-resolution structures of protein complexes to tools capable of achieving atomic and quasi-atomic resolution for complexes that nobody could solve with any other technique.
This incredible leap forward has been made possible through the introduction and adoption of new tools, in particular direct electron detectors (DED), ultra-stable cryo-microscopes, such as the Titan Krios and the adoption of new SW for automatic data collection and processing.
Cryo-EM benefits of specific advantages, with respect to other structural biology techniques such as NMR and X-ray diffraction:
. Crystallization or isotopic labelling is not needed.
. The amount of sample required is two orders of magnitude lower.
. Different functional conformation of a protein complex may be revealed.
Cryo-EM has proven to be a very useful technique to be integrated with X-ray and NMR for structure-based drug design Thus it is no surprise that many structural biology groups all over the world are seeking access to this technology in order to find answers to their most relevant biological questions. Nevertheless most users that are new to the field of cryo-EM are struggling to overcome the adoption barrier that this technique may pose in terms of: sample preparation and screening, automatic data acquisition and progressive users training.
In this presentation we will present how the fast pace of cryo-EM growth is going to revolutionize the structural biology landscape. In addition, the latest Thermo Fisher Scientific technologies/solutions will be introduced:
- The cryo-EM SPA workflow with the recently introduced electron microscopes Krios G3i and Glacios - today the most economical and efficient Cryo-EM solutions for getting to the 3D structure of protein molecules.
- The recent advances in electron cryo-tomography with the cryo focused-ion-beam, an application for visualization of biological structures in their native, cellular context at the molecular-scale.
- The new development of mED (Micro electron Diffraction): a technology that holds the promise to solve the high resolution 3D structure of proteins crystallized into very small., an interesting cost effective alternative for XFEL.
We cordially invite you to the 7th MedInProt Conference held at ELTE TTK Eötvös lecture hall on Saturday, April 21, 2018 starting at 9 AM.
Winners of the Synergy VI., VII. and the Feasibility Study Program shall present their joint research at the conference. In addition, topic covers in cryo-EM Mihály Pósfai and Ferenc Vonderviszt will give lectures.
The conference will end with sweepstakes and poster discussions.
All interested are invited and welcome!
Az MTA Szerves és Biomolekuláris Kémiai Bizottság és a Kajtár-Hollósi Alapítvány tisztelettel meghívja Önt, barátait, tanítványait és minden kedves érdeklődőt a soron következő „Bruckner termi előadásra” és az Alapítvány emlékülésére
Időpont: 2017. december 1. péntek 14.00 óra
Helyszín: ELTE TTK Kémiai Épület, (1117 Budapest, Pázmány P. sétány 1/a)
The Molecular Frontiers Symposium was held on September 14-15. 2017, at Eötvös Loránd University.
Presented at the conference:
September 4. 2017, 4 p.m., MTA TTK
Molecular Frontiers (www.molecularfrontiers.org) is an international network formed by researchers and professors. This organization focuses on highlighting the importance of molecular sciences in a wide range of society, thus attracting young people towards science. Molecular Frontiers Symposia, organized for the upper purpose gathers leading scientists of the Globe, usually to discuss the topic of a current global problem. The actual issue and the attendees of these events are decided by the Scientific Committee (many members of which are Nobel laureate scientists); thus young student (age usually from 14 to 25) and other participants have the opportunity to meet the top researchers of the field. Further information regarding past events at: http://www.molecularfrontiers.org/symposia).
On each symposia 10 to 12 researcher hold lectures many of whom have received a Nobel Prize. On the demonstrating photo a group picture of the 2015 event in Göteborg can be seen. More than 1000 students and researchers attend Molecular Frontiers events, moreover, thanks to the recently initiated live broadcasting via internet, Molecular Frontiers Symposia are becoming rapidly expanding international events.
We believe that the Hungarian organization of such a prestigious event not only furthers molecular science’s popularity and recognition among the young, but also promotes the international (mostly inter EU) recognition of Hungarian research, thus, fulfilling an important national interest. The currently chosen topic shall follow the theme of an actual Hungarian program known as MedInProt initiative, with the purpose of assisting the communication among scientists working in the fields of protein sciences and pharmacology. The goal of MedInProt and Molecular Frontiers is to efficiently support the more successful integration of Hungarian scientists into the international network.
Vesse föl új és eredeti ötletét, akár még ma, nyújtsa be támogatási igényét, és a MedInProt kuratóriuma rövid megfontolás után, ha a kezdeményezést méltónak találja, azonnal támogatja is. „first come first served”.
Figyelmébe ajánlom „Megvalósíthatósági” programunkat (http://medinprot.chem.elte.hu/hu/palyazatok/uj-palyazatok)
- a szinergiában résztvevő kutatók közül legalább az egyik a 4 központi intézmény (ELTE, BME, MTA-TTK, SE) valamelyikében legyen főállású közalkalmazott,
- a javaslat világosan mutasson rá arra, hogy Ön és társa(i) az ad hoc támogatásnak köszönhetően hogyan jutnak közelebb hosszú távú a közös munkához és egy közös pályázáshoz akár hazai, akár nemzetközi fórumon.
Jelen „ad hoc” támogatás volumene:
200 eFt <= x <= 2M Ft (eltérés csak kiemelten indokolt esetben lehetséges)
Jelen „ad hoc” támogatás pályázási határideje:
2017. május 10.
Küldje el a firstname.lastname@example.org e-mail címre.
Budapest, 2017. május 2.
a MEDinPROT kuratórium elnöke
The sixth Conference of the MedInProt Protein Science Research Synergy Program was held on April 22. 2017, at Eötvös Loránd University.
Studying in vivo effect of cell-penetrating inhibitor peptide conjugate on protein-protein interaction
Gábor Mező: Development of bioconjugates and their modules for targeted treatment of cancers with high mortality rate
There is increased interest in peptide based targeting moieties for drug delivery in targeted tumor therapy, that recognize the specific or overexpressed receptors on tumor cells with high selectivity. Despite there are promising results with peptide – drug conjugates, their application in combination similarly to chemotherapy might have benefits. For this purpose we would like to develop molecular libraries which modules (drugs, homing peptides and bifunctional linkers) can be attach to each other in high combination to develop numerous conjugates suitable for targeted tumor therapy.
Beáta Vértessy: K-RAS oncogen mutations: structure-based allele specific inhibitor design
Balázs Győrffy: Investigating pediatric solid tumors with worst prognosis
Malignant diseases represent the leading cause of death for those below 14 years of age. While treatment of hemato-oncologic malignancies can almost completely heal a patient, half of patients with a solid tumor are lost within ten years. In our project we will gain new insights into the development of solid tumors by linking molecular and epidemiologic data.
We cordially invite you to the 6th MedInProt Conference held at ELTE TTK Eötvös lecture hall on Saturday, April 22, 2017 starting at 9 AM.
Winners of the Synergy V Program and the Grant Facilitating Access to Instruments Program shall present their joint research at the conference. In addition, NVKP winners Gábor Mező (ELTE), Beáta Vértessy (BME) and Balázs Győrffy (MTA) will give lectures.
The conference will end with sweepstakes and poster discussions.
All interested are invited and welcome!
The Protein Science Excellence Collaboration Program (MedInProt Program), sponsored by the Hungarian Academy of Sciences shall be expanded from 2017. Full-time researchers or professors working in an institute of the Academy’s research network or at any government financed institution of higher education may join the cooperation of ELTE, BMGE, SE and MTA-TTK employees. In the past three years several successful collaborations have started thanks to MedinProt. Due to the success the board has decided to expand the network of institutes from Spring 2017, thus, reaching a further step in the organizing a network of specialties.
MedinProt is a unique and essential initiative in Hungary with the following aims:
- Connecting, organizing and strengthening different protein science subfields
- Introducing the practice of competitive cooperation
- Catalyzing synergism among different fields of research
- Reinforcing and financially supporting the collaboration of acknowledged researchers
The Program mainly supports the following areas (from 2014):
1) The Synergy Program, announced every semester, provides a moderate, but regular financial support for the selected projects, in which 2-4 postdoctoral researchers can work together in solving a new and promising problem of protein science
2) The Access to Instruments Program, supports pairs of researchers whose research progress largely depends on recordings obtained by certain instruments, therefore, the fund covers the fees of using dedicated large instruments (X-ray, NMR, ESR, MS, etc.)
3) The Instrument Purchase Program is to support pairs of researchers whose scientific progress could significantly be furthered by the purchase of a large instrument (3 million HUF tops).
4) In the Mentor Program protein scientists with different backgrounds and positions may work together for a couple of weeks (2 months tops). The education of the PhD student/postdoc who is less experienced in the given field is supervised and supported by a chosen “mentor”.
5) The Summer School Program provides the opportunity to learn any kind of protein science related technique or to expand ones knowledge in the use of a large instrument, during few-day technical workshops for small groups.
6) The Feasibility Program offers a one-time support (1 million HUF tops) for protein science-related preliminary studies necessary for the application to large national or international grants
Conditions of the application:
1) potential applicants are required to register on MedinProt’s homepage
2) New candidates should apply together with researchers of the previously eligible institutes (ELTE, BMGE, SE and MTA-TTK), they must join the research of one of the upper institutes, thus, strengthening the national synergy.
In each case, submitted applications shall be reviewed by a board of eight members.
March 8. 2017. 4 p.m. MTA TTK Kisterem
February 8. 2017. 4 p.m., MTA TTK Kisterem
We congratulate the research groups of Andrea Bodor (structural chemistry) and László Nyitray (biochemistry) for their success, since their work appeared on the cover page of ChemBioChem. It is a great pleasure and honor for MedInProt to support such an excellent and productive collaboration.
December 14. 2016. 9.00 a.m. MTA TTK Nagyterem